Fig. 3
In TC11 tumors, RT induces dynamic changes in markers for a type I IFN response and immune susceptibility, and immune cell subpopulations. A Schematic of treatment timeline. TC11 tumors in the caudal mammary fat pads were treated ± a single dose of RT (8 Gy) 28 days (4 weeks) after tumor cell transplantation (tumors about 150mm3). Cohorts of mice were euthanized 5, 10, 15, and 22 additional days after RT, and the tumors were harvested for qPCR and flow cytometry analyses. B Relative levels of transcripts for genes associated with a type I interferon response (Ifnβ, Mx1, Oas2, Oas3) and immune susceptibility (Pdl1, Mhc-1) are shown. (Mean ± SEM, n = 5). A twofold increase (dotted line) or greater is considered significant. C, D Relative proportions of intratumoral CD45+ immune cells that expressed Ly6G+Ly6C+(C) or CD8+CD4− (D), analyzed by flow cytometry. (Mean ± SEM, n = 5). The mean levels ± SEM in tumors which were not irradiated are shown by the shaded lines