Fig. 6

The tumor microenvironments of lung and mammary fat pads differ, but are minimally altered by Rhamm-loss. A MDA-MB-231 RHAMM^+/+ tumor cells were exposed to H₂O₂, and the effect on STAT1 activation was quantified as the number of cells/field with nuclear STAT1 immunofluorescence. H₂O₂ significantly increases STAT1 activation. Values are the Mean and S.E.M. n = 5 fields. B Reactive oxygen species (ROS/NOS) levels in Wildtype and Rhamm^−/− primary and metastatic tumors were quantified using ELISA as described in Methods. Results show that levels are similar in tumors of both genotypes. Notably ROS levels are higher in lung vs. primary tumors from both genotypes. Values are the Mean and S.E.M. of n = 4 biological replicates and 3 technical replicates *p < 0.05, **p < 0.01. C IFNG protein levels were measured by antibody array in Wildtype and Rhamm^−/− lungs with metastases as described in Methods. IFNG protein is highly variable in both genotypes but not significantly different. Values are the median and Std. Dev. n = 3 replicates. D TGFB protein was quantified by IHC in primary and lung tumors. TGFB levels are significantly higher in Rhamm^−/− lungs than in Wildtype counterparts. Values are the Mean and S.E.M. of n = 5 lungs/genotype