Fig. 2

In human breast cancer tissue, mitochondrial CA5A and CA5B are ubiquitously expressed across cell types, whereas the non-mitochondrial carbonic anhydrase isoforms predominate in cancer epithelial and endothelial cells, except for CA7 expressed solely by cancer-associated fibroblasts and myeloid cells. A Overall carbonic anhydrase expression intensities illustrated as t-SNE plot (left) with corresponding clustered cell types (right). B Dot plot showing the cell type-specific pattern of carbonic anhydrase expression in human breast cancer. The reported single-cell sequencing data cover 24,489 cancer epithelial cells, 6573 cancer-associated fibroblasts, 7605 endothelial cells, 35,214 T cells, 3206 B cells, and 9675 myeloid cells. Data were extracted from the online Single Cell Portal hosted by the Broad Institute [46]. C Dot plot showing the protein expression levels of carbonic anhydrases across 294 breast cancer samples. Data were extracted from the online Proteomic Data Commons portal hosted by the National Cancer Institute. CA, carbonic anhydrase