Fig. 1

Macrophage-mediated induction of MenaINV expression via Notch and NF-κB cooperation. A MenaINV mRNA expression in MDA-MB-231 (231) cells co-cultured with or without BAC2.1F macrophages (Mac) and with or without 10 ng/ml TNFα for 4 h. B MenaINV mRNA expression in 231 cells co-cultured with or without Macs, NF-κB inhibitor (DHMEQ), or Notch/γ secretase inhibitor (DAPT) for 4 h. C Model of potential Notch1 and NF-κB signaling crosstalk leading to enhanced transcriptional activity at the ENAH (Mena) promoter. The released Notch intracellular domain (NICD—shaded in gray) can bind to the transcription factors in the NF-κB signaling pathway and prevent their nuclear export, allowing for enhanced and sustained transcriptional activation of target genes and alternative splicing. The bars in A and B represent average fold change MenaINV mRNA compared to control (231 cells), ± S.D. The data were analyzed using a one-way ANOVA with Tukey’s multiple comparisons test.*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, n.s. = not significant. All experiments were repeated at least three times