Fig. 6

The effect of attenuated TGFB signalling on abundance and location of iNOS-positive cells within the mammary stroma. Representative iNOS antibody staining in the stroma around the ductal (a–c) and alveolar (d–f) epithelium. Examples of iNOS-positive stromal macrophages are indicated by arrowheads. The number of iNOS -positive (g) cells within the stroma was calculated in the mammary glands from doxycycline-treated single transgenic control Cfms-rtTA (n = 10; a, d) mice, TetO-TbrII (n = 8, b, e) mice and double transgenic Cfms-TbrII (n = 12; c, f) mice. Data are presented as mean + SEM with statistical analysis using a one-way ANOVA test, *p < 0.05, **p < 0.01, ***p < 0.005