Fig. 1

Transcriptional and CNA profiles of ER-positive local recurrences. a Unsupervised hierarchical clustering and heatmap (red = high relative expression, blue = low relative expression) on normalized gene expression values from patient-matched pairs (P1 = primary, R1 = recurrence). Clinical ER and HER2 status (black = negative, green = positive, gray = unknown), tissue source site (purple = Pitt, yellow = Charite), and tumor type (blue = primary, red = recurrence) are indicated. Delta symbol shows distinct clustering of ERLR_03_R1 away from its matched primary, ERLR_03_P1. b Heatmap of copy number ratios from patient-matched pairs. Redder regions indicate regions of copy number gain and bluer regions indicate regions of loss. c Correlation between ERBB2 DNA copy number calls and normalized expression values