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Table 3 Peptide candidates selected for the MRM assay

From: Shotgun proteomics coupled to nanoparticle-based biomarker enrichment reveals a novel panel of extracellular matrix proteins as candidate serum protein biomarkers for early-stage breast cancer detection

Gene

Peptide sequence

Precursor m/z

Precursor charge

FERMT3

VFVGEEDPEAESVTLR

888.93

2

VVLAGGVAPALFR

635.38

2

ACTG1

GYSFTTTAER

566.77

2

ACTG1/POTEF

AGFAGDDAPR

488.73

2

AVFPSIVGRPR

599.86

2

ACTN (ACTN1/ACTN4)

VGWEQLLTTIAR

693.89

2

LASDLLEWIR

608.34

2

GP1BB

LSLTDPLVAER

607.34

2

RAP1 (RAP1A/RAP1B)

LVVLGSGGVGK

493.31

2

SKINVNEIFYDLVR

570.65

3

SALTVQFVQGIFVEK

833.46

2

TUBA

EIIDLVLDR

543.31

2

LISQIVSSITASLR

744.44

2

TUBA

VGINYQPPTVVPGGDLAK

913.00

2

TUBB1

GASALQLER

472.76

2

EVDQQLLSVQTR

708.38

2

TUBB (TUBB1/TUBB3/TUBB6)

FPGQLNADLR

565.80

2

ITGB3

SKVELEVR

480.28

2

PFN1

STGGAPTFNVTVTK

690.36

2

TFVNITPAEVGVLVGK

822.47

2

CFL1

LGGSAVISLEGKPL

670.89

2

BMP1

LNGSITSPGWPK

628.84

2

LTF

DGAGDVAFIR

510.76

2

ITGA2B

VAIVVGAPR

441.28

2

VYLFLQPR

518.30

2

THBS1

SITLFVQEDR

604.32

2

GFLLLASLR

495.31

2

FLNA; FLN1

ANLPQSFQVDTSK

717.86

2

YGGQPVPNFPSK

645.83

2

SPFSVAVSPSLDLSK

767.41

2

MYH9

ALELDSNLYR

597.31

2

HPSE

FLILLGSPK

494.32

2

TDFLIFDPK

548.29

2

AHSG

HTFMGVVSLGSPSGEVSHPR

699.68

3

TLN1

LAQAAQSSVATITR

708.89

2

ILAQATSDLVNAIK

728.92

2

GLAGAVSELLR

543.32

2

TLN2

VMVTNVTSLLK

610.85

2

SIAAATSALVK

516.31

2

MST1

VVGGHPGNSPWTVSLR

831.94

2

LIMS2

VIEGDVVSALNK

622.35

2

  1. Peptides were selected using the following criteria: highest number of identified peptides and intensities from discovery dataset, presence of at least 3 high abundant − b and/or − y product ions, highest ESP scores and scored transitions in the PeptideAtlas, no Met residues, and + 2 or + 3 charge state precursor ions. The protein isoforms are described in parenthesis
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Breast Cancer Research

ISSN: 1465-542X

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