Fig. 7
From: Sunitinib facilitates metastatic breast cancer spreading by inducing endothelial cell senescence
The premetastatic niche environment induced by sunitinib promoted MBC to metastasize to the lungs. a The relative mRNA levels of Bv8, S100a8, S100a9, and MMP9 were measured by real-time RT-PCR (n = 5). b Balb/c female mice were pre-treated with sunitinib (100 mg/kg/day) or saline for 2 weeks, followed by orthotopic inoculation with 1 × 106 transfected 4T1 cells. The tumor volume was measured and presented as scatter plots with mean ± SEM (n = 10). c Representative images of the lungs with metastatic tumor colonies. d, e The number of surface metastatic colonies and the area of the metastatic lesion were quantified and presented as scatter plots with mean ± SEM (n = 10). e Kaplan-Meier survival analysis of the mice pretreated with sunitinib or saline. f Lung metastatic ability assay using patient-derived xenografts (n = 8). Significance was assessed by unpaired t test with Welch’s correction (b, d) and log-rank test (e)