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Fig. 3 | Breast Cancer Research

Fig. 3

From: Trastuzumab-induced upregulation of a protein set in extracellular vesicles emitted by ErbB2-positive breast cancer cells correlates with their trastuzumab sensitivity

Fig. 3

Liquid chromatography-tandem mass spectrometry analysis of proteins in extracellular vesicles emitted by ErbB2-postive human breast tumor cells before and after trastuzumab treatment. BT-474 or BT-474TR cells were treated (+) or not (−) with 5 μg/ml trastuzumab (TZ) in 3D culture for 72 h. Extracellular vesicles were isolated from the cell media by ultracentrifugation. EV protein content was digested by trypsin and subjected to LC-MS/MS. The raw data were matched against the latest human SwissProt protein database using Mascot search engine. Subsequently, label-free TIC quantification was performed by Scaffold Q+ software. About 400 proteins (protein threshold > 95.0%, peptide threshold > 95.0%, and a minimum of two unique peptides per protein) were identified in the EVs. The signal intensities for each of the indicated proteins are expressed in TIC units. The data for ITB1 (a), GNAS2 (b), GNA13 (c), CTL2 (d), and RAB10 (e) are shown. The data represent the average of three independent experiments plus the SE. *p < 0.05

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Breast Cancer Research

ISSN: 1465-542X

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