Fig. 2

Net1 deletion delays mammary gland hyperplasia and inhibits proliferation. a Representative examples of inguinal mammary gland whole mounts from Net1^+/+,PyMT and Net1^−/−,PyMT mice at 5 weeks of age. b Quantification of hyperplastic mammary gland area at 5 weeks in genotypes shown. Six mice per genotype analyzed. c Representative examples of Ki67 staining in hyperplastic mammary gland sections from mice at 5 weeks. d Quantification of Ki67-positive cells in hyperplastic mammary gland sections at 5 weeks. Five independent regions within each sample quantified, 3–4 animals per genotype. e Representative examples of PyMT expression in hyperplastic mammary gland sections at 5 weeks. f Quantification of PyMT staining in mammary gland sections. Six independent regions within each sample quantified, 3 animals per genotype. g Representative examples of Ki67 staining in tumors at 14 weeks. h Quantification of Ki67-positive cells in 14-week tumors. Ki67 staining quantified as percent positive area divided by total area. Five independent regions within each sample quantified, 6 animals per genotype. Errors are standard error of the mean. *P < 0.05; **P < 0.01; ***P < 0.001. Net1 neuroepithelial transforming gene 1, n.s. not significant, PyMT polyoma middle T antigen