Fig. 1From: Pathomimetic avatars reveal divergent roles of microenvironment in invasive transition of ductal carcinoma in situMyoepithelial cells (MEPs) reduce volume and malignant phenotype of ductal carcinoma in situ (DCIS) xenografts. MCF10.DCIS (DCIS) cells, N1ME cells (MEPs), and/or WS-12T (cancer-associated fibroblasts [CAFs]) were implanted under the renal capsule or orthotopically within the mammary fat pad of female severe combined immunodeficiency mice and evaluated after 8 weeks. a Representative orthotopic xenografts. Scale bar = 5 mm (top row); hematoxylin and eosin (H&E) staining; scale bar = 100 μm (middle row); and Masson’s trichrome staining for collagen (blue); scale bar = 100 μm (bottom row). b Volume of orthotopic xenografts (n = 3–8). c Representative renal xenografts. Scale bar = 5 mm (top row); H&E staining; scale bar = 100 μm (middle row); and Masson’s trichrome staining for collagen (blue); scale bar = 100 μm (bottom row). d Volume of renal xenografts (n = 6, except for CAFs alone, which were n = 2 and not used in statistical analyses). Data are presented as box-and-whisker plots where the box represents the interquartile range and whiskers represent minimum and maximum values. * p ≤ 0.05; **** p ≤ 0.0001 as determined by one-way analysis of variance and Bonferroni’s multiple comparisons test. Immunostaining and immunoblotting results for basal markers are shown in Additional file 1: Figure S1Back to article page