Fig. 7From: SMARCE1 regulates metastatic potential of breast cancer cells through the HIF1A/PTK2 pathwaySMARCE1 orchestrates chromatin remodeling of PTK2 promoter in detached cells. a Overlapping primer designed for the nucleosome scanning assay and their amplicon sizes. The location of each primer set is given relative to the transcription start site (TSS). b Significant nucleosomal displacement is observed on PTK2 promoter (from −802 to −492 bp) in LM-EV cells after 0.5, 1, and 2 hours under suspension culture (vs. adherent cells), but not in LM-SMARCE1-KD cells. Results from three independent experiments were presented as means ± SE. EV empty vector, KD knockdown, LM lung metastatic cell line derived from MDA-MB-231Back to article page