Fig. 1
From: COX-2 modulates mammary tumor progression in response to collagen density
Cyclooxygenase-2 (COX-2) and prostaglandin E2 (PGE2) levels are elevated in collagen-dense tumors. a, b Immunofluorescence images of COX-2 or PGE2 (magenta) counterstained with 4',6-diamidino-2-phenylindole (DAPI) (green); ×20 objective, scale bar = 100 um. Composite is zoomed-in image of area demarked by the yellow window; scale bar = 15 um. c, d Quantitation of several images as shown in a and b. Box plots are overlaid with violin plots to indicate differences in the density distribution of data points. Raw data are depicted. Epi epithelium, Str stroma. Quantitative values represent the number of positive stained cells divided by the total number of cells in that compartment. c COX-2 levels are elevated in tumor and stromal cells from collagen-dense tumors in PyMT/Col1a1 compared to tumors arising in PyMT mice. d PGE2 levels are moderately elevated in PyMT/Col1a1 tumors in cells in the stromal compartment; *p < 0.05; n = 5 mice; at least 8 image fields analyzed per 2–3 tumors per animal