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Table 3 Primary cells confirm the heterogeneity of the tissue

From: Selective isolation and characterization of primary cells from normal breast and tumors reveal plasticity of adipose derived stem cells

 

Cell types

EPCAM

CD49f

CD24

MUC1

KRT8

KRT18

KRT14

ACAT1

VIM

Gene expression signature according to [9, 17, 40]

luminal 1

high

--

+

+

high

high

--

--

low

luminal 2

high

+

+

+

high

high

--

--

+

basal-like

low

+

--

--

low

low

+

high

high

mesenchymal

--

+

--

--

low

low

low

low

high

 

cell lines

         

Gene expression of IFDUC1 cell lines compared to of NORMA1-4

MEC of IFDUC1

low 2.8 × lower

+ 1.4 × higher

+ 2 × higher

--

low 1.8 × lower

low 2.6 × lower

+

equal

high 2.5 × higher

high 1.3 × higher

MES of IFDUC1

+

1.7 × higher

low 1.5 × lower

--

high 7.8 × higher

low 5.8 × lower

+

--

high 7.7 × higher

high 10 × higher

ADSC of IFDUC1

--

low 1.8 × lower

--

low 1.8 × lower

low 1.5 × lower

+ equal

--

high 1.6 × higher

high 1.3 × higher

  1. For isolated primary cells the lower/higher values correspond to the mean of normal mammary cells (NORMA)1-4 for mammary epithelial cells (MEC), mesenchymal cells (MES) or adipose-derived stem cells (ADSC). Fold-changes (×) represent lower, equal or higher expression of invasive inflammatory ductal carcinoma (IFDUC)1 cell lines compared to NORMA1-4 mean values