Fig. 2

CD24⁺ cells display in vivo aggressiveness in both wild-type (WT) and MKR mice. a 1*10³ CD24⁻ and CD24⁺ cells were seeded and grown in full medium for 2 days, and relative proliferation rate between the cells was determined using the Cyquant proliferation assay. b Representative photomicrographs of a tumorsphere (>50 um diameter) grown from CD24⁻ and CD24⁺-single cells in non-adherent culture for 5 days (upper panel). Percentage tumorsphere formation efficiency (TFE %) comparison between CD24⁻ and CD24⁺ (lower panel). The Mann-Whitney test was performed to compare CD24⁻ and CD24⁺ cells (*p <0.05). c CD24⁻ and CD24⁺ cells were injected into the fourth mammary fat pad of 8-week-old virgin WT and MKR mice (50,000 cells/mouse), and tumor volume was measured during a 5-week period. d Tumor weights were measured at necropsy. The Mann-Whitney test was performed to compare CD24⁻ and CD24⁺ tumors (*p <0.05, and to compare tumors in WT and MKR mice (^# p <0.05)