Figure 3

High-throughput screen for genes regulating mammary epithelial cell growth. (A) Summary of screen and follow-up assays leading to the identification of 47 candidate target genes. (B) Ranked distribution of growth effects for small interfering RNA (siRNA) pools utilized in the siRNA screen, normalized to the Lipofectamine 2000 (LF2K) reagent-alone control condition. Pooled siRNAs with a statistically significant effect that reduced growth to 25% or less of the control condition are highlighted in blue. (C) Ranked distribution of growth effects for siRNA pools utilized in the secondary screen targeting genes on the plasma membrane and in the extracellular space. Pooled siRNAs with a statistically significant effect in 184-hTERT-L9 and/or 184-hTERT-E11 cells that reduced growth to 25% or less of the control condition are highlighted in red. EGF, Epidermal growth factor; RT-QPCR, Quantitative RT-PCR.