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Figure 4 | Breast Cancer Research

Figure 4

From: Profiling gene promoter occupancy of Sox2 in two phenotypically distinct breast cancer cell subsets using chromatin immunoprecipitation and genome-wide promoter microarrays

Figure 4

Mammosphere formation is dependent on novel sex-determining region Y (SRY)-box binding protein-2 (Sox2) target Muc15. (A) Western blot analysis of Muc15 in MCF7 reporter unrepsonisve (RU) and reporter responsive (RR) cells. (B) MCF7 unsorted cells were treated with 40 nM of Muc15 siRNA for 72 hours before seeding into mammosphere culture. Mammospheres were counted on day 7, and subsequently trypsinized and counted after trypan blue incubation. Accompanying western blot shows Muc15 knockdown efficiency.

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Breast Cancer Research

ISSN: 1465-542X

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