Skip to main content
Figure 3 | Breast Cancer Research

Figure 3

From: Profiling gene promoter occupancy of Sox2 in two phenotypically distinct breast cancer cell subsets using chromatin immunoprecipitation and genome-wide promoter microarrays

Figure 3

Sex-determining region Y (SRY)-box binding protein-2 (Sox2) can upregulate target gene transcripts in reporter responsive (RR) cells only and not reporter unresponsive (RU) cells. (A) MCF7 RU and RR cells were transfected with 3 μg of pcDNA3-Flag-Empty vector (EV), or pcDNA3-Flag-Sox2 (Sox2) and harvested for mRNA after 72 hours. Quantitative (q)-PCR analyses were performed using primers designed against MCF7 RR chromatin immunoprecipitation promoter microarray chip analysis (ChIP-chip) targets. Accompanying Sox2 qPCR analysis and Flag western blot shows transfection efficiency. (B) Western blot showing Sox2 knockdown efficiency in MCF7 RU and RR cells after 72-hour 20-nM scrambled or Sox2 siRNA treatment. q-PCR mRNA transcript analysis of MCF7 RU and RR cells after 72-hour 20-nM scrambled or Sox2 siRNA knockdown examining RR ChIP-chip genes in MCF7 RU and RR. (C) q-PCR analysis of MCF7 RU and RR cell PROM1 (CD133) transcripts after 16-hour treatments with dimethyl sulfoxide vehicle control or 10, 20, 30, or 40 nM Triptolide.

Back to article page

Breast Cancer Research

ISSN: 1465-542X

Contact us