Figure 2From: Subtype-specific overexpression of the Rac-GEF P-REX1 in breast cancer is associated with promoter hypomethylationDNA methylation status of the PREX1 promoter in human mammary cell lines. Panel A. Schematic representation of the PREX1 promoter region studied with the Hs_PREX1_04_PM PyroMark CpG assay (Qiagen), and representative pyrosequencing experiments for MCF-10A, MDA-MB-231, and BT-474 cell lines. Panel B. Pyrosequencing results of the PREX1 methylation in breast cancer cell lines. Results are expressed as mean ± S.D. of the percentage of methylation for the six CpG dinucleotides measured by the pyrosequencing assay. Panel C . The PREX1 promoter methylation levels for probe cg24364574 across 51 cancer cell lines from the Infinium HumanMethylation27 BeadChip array (GEO accession number: GSE42944). (***, P <0.0001).Back to article page