Figure 4
Apoptosis induced by bis-aziridinylnapthoquinone (AZ-1). BC-M1 cells were treated in 24-hour culture with (a) dimethylsulfoxide control, (b) 0.2 μM AZ-1, (c) 1.0 μM AZ-1, or (d) 2.0 μM AZ-1 before propidium iodide staining and analysis of the DNA content. Apoptosis is apparent from the large population of cells with increased DNA content in the sub-G1 area (M1 area). FL2-H, fluorescence two-peak high.