Figure 1

Immature dendritic cells (DCs) capture killed breast cancer cells. (a) HCC1806 cells are treated with treated with cycloheximide (50 mg/ml) in a sensitization step. After 2 hours at 37°C, anti-Fas antibody (CH11; Immunotech) is added at a concentration of 1 mg/ml and the cells are incubated for the indicated period of time. (b) 7-Aminoactinomycin D (7AAD)-labeled killed breast cancer cells are incubated for 1 hour at 37°C with CD1a-labeled immature monocyte derived DC at a 1:1 ratio. The capture is determined by flow cytometry (the double-positive CD1a/7AAD represents the DCs that have captured killed breast cancer cells) and confirmed by confocal microscopy (c). Propidium iodide.