Figure 2
From: Molecular analysis of metastasis in a polyomavirus middle T mouse model: the role of osteopontin
Northern blot of cell lines and tumors from Db and Met. RNA was isolated from Met and Db tumors and from Met and Db cell lines, and probed on a northern blot using 32P-labeled mouse osteopontin (OPN) cDNA. The 18s ribosomal RNA was used as a control. The 1.6 kb OPN transcript level is fourfold higher in the Met tumor than in the Db tumor, and is 3.5-fold higher in the Met cell line than in the Db cell line.