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Table 2 Engraftment capacity of different cell populations sorted from cryopreserved tumor cells

From: Malignant phyllodes tumors display mesenchymal stem cell features and aldehyde dehydrogenase/disialoganglioside identify their tumor stem cells

BC-P007

4 × 104

2 × 104

1 × 104

5 × 103

4 × 103

1 × 103

500

100

50

20

Frequency〒

Distribution#

ALDH+

 

3/8

  

3/8

1/3

1/7

   

1:2.0 × 104

75%

ALDH-

 

0/4

  

0/6

1/7

0/3

0/4

  

1:1.1 × 105

25%

ALDH+/GD2+

 

2/2

  

3/3

6/10

 

5/10

2/20

0/3

1:6.1 × 102

 

ALDH-/GD2-

0/4

 

0/1

0/8

2/10

0/15

    

1:1.3 × 105

 

ALDH+

 

3/8

  

3/8

1/3

1/7

   

1:2.0 × 104

 

ALDH+/GD2+

 

2/2

  

3/3

6/10

 

5/10

2/20

0/3

1:6.1 × 102

 

BC-P515

1 × 10 5

2 × 10 4

1 × 10 4

 

4 × 10 3

1 × 10 3

500

100

 

50

Frequency 〒

Distribution #

ALDH+

 

1/4

0/3

 

1/4

3/9

 

1/5

  

1:2.0 × 104

96%

ALDH-

 

0/4

0/3

 

0/2

0/9

    

1:>4.2 × 105

4%

ALDH+/GD2+

 

1/3*

7/10

  

4/5

0/1

2/7

 

1/2

1:7.1 × 103

 

ALDH-/GD2-

0/1

0/2

1/9

 

0/4

0/10

    

1:2.5 × 105

 

ALDH+

 

1/4

0/3

 

1/4

3/9

 

1/5

  

1:2.0 × 104

 

ALDH+/GD2+

 

1/3

7/10

  

4/5

0/1

2/7

 

1/2

1:7.1 × 103

 
  1. BC-P007 and BC-P515 cryopreserved tumor cells from patient-derived xenografts of PT were sorted by FACS into ALDEFLUOR-positive, ALDEFLUOR-negative or ALDEFLUOR and GD2 double positive and double negative cells. Varying numbers of the sorted cells were injected in fat pads of NOD-SDID mice to evaluate their tumor-initiating potential. The frequency of cancer-initiating cells was analyzed using ELDA software.
  2. *:Including one of three mice died the day after tumor injection.
  3. #:The distribution of tumor-initiating cells among the BC-P007 and BC-P515 ALDH + and ALDH- cells.
  4. 〒:Frequency was calculated by ELDA software, although the P-value for likelihood ratio test of single-hit model in each group was less than P = 0.5.