Figure 6From: Pregnancy-associated breast cancers are driven by differences in adipose stromal cells present during lactationCrabp1 regulates CAF phenotype. (A) Tumor growth curve of mice injected at eight weeks of age with SUM159 cells co-mixed with CAF-L cells (n = 10 per group). P value indicates significance compared to SUM159 cells alone. Data are means ± SEM. (B) Quantitative RT-PCR of crabp1 in CAF-L cells transduced with shRNA for crabp1 or scrambled control. Data are presented as average 2(-ΔΔCt ± SEM; n = 3. (C) Representative image of Oil Red O staining and quantification of CAF-L cells transduced with shRNA for crabp1 or scrambled control and exposed to adipocyte differentiation media. Data are presented as average absorbance ± SEM. Untreated cell absorbance is set to one; n = 3 experiments. Scale bar = 100 μm. (D) Representative image of Oil Red O staining and quantification of NIH3T3 cells treated with shRNA for crabp1 or scrambled control and exposed to adipocyte differentiation media. Data are presented as average absorbance ± SEM. Untreated cell absorbance is set to one; n = 3 experiments. Scale bar = 100 μm. (E) Tumor growth curve of mice injected at eight weeks of age with SUM159 cells co-mixed with CAF-L cells transduced with shRNA for crapb1 or scrambled control (n = 10 per group). P value indicates significance compared to shScr-CAF-L cells. Data are means ± SEM. (F) Representative H & E images of SUM159 tumors co-mixed with CAF-L cells. V = vessel; MG = mammary gland duct. Scale bar = 100 μm. (G) Representative images and quantification of the percentage of centralized tumor necrosis in SUM159 cells co-mixed with CAF-L cells transduced with shRNA for crapb1 or scrambled control (n = 5 per group). (H) Schematic of phenotypic modulation of adipocytes under physiologic (normal development) and pathologic (breast cancer progression) conditions. CAF-L, carcinoma-associated fibroblast-like; crabp1, cellular retinoic acid binding protein-1; SEM, standard error of the mean.Back to article page