Figure 6
From: Embryonic mammary signature subsets are activated in Brca1-/- and basal-like breast cancers
Effects of SOX11 knockdown on cell proliferation and viability of breast cancer cells. (A) SOX11 expression levels in BT474 cells transfected with either SOX11 or control siRNAs. SOX11 was detected with immunoblotting. (B) BT474 cell number, represented as measured by absorbance by using PrestoBlue cell-viability reagent, after transfection with SOX11 or nontargeting siRNAs at daily intervals. Values represent mean ± SEM for three different experiments. (C) Change in percentage of viable cells was assessed by using PrestoBlue cell-viability assay of BT474 cells 72 hours after transfection with SOX11 siRNAs compared with control siRNA. Values represent mean ± SD for three different experiments; *P < 0.001, compared with the control. (D) Caspase-3 and cleaved caspase-3 levels detected by immunoblotting of lysates of adherent and floating BT474 cells transfected with either SOX11 or control siRNAs. (E) After transfection with either SOX11 or control siRNA, adherent BT474 cells were collected and stained with 7AAD. Cell-cycle phase was determined with FACS analysis. Values represent mean ± SD for four independent experiments. *P < 0.05; and **P < 0.01 compared with the control.