Figure 7

MMP-13 expression is associated with the presence of TRAP positive cells within bone marrow and tumour. A. Histology, immunohistochemical staining for MMP-13 and pan CK, and TRAP staining were performed on femurs injected with PBS, wild type, Scrambled and MMP-13 shRNA MDA-MB-231 cell clones. Representative images are shown for each conditions. The number of TRAP positive cells at tumour/bone interface (B) and in the bone marrow infiltrated by tumour cells (C) are reported. Six sections of 10 × fields of four mice for each treatment were examined. Data are expressed as mean ± SD. *P < 0.05. Scale bars = 100 μm.