Figure 4
From: Estrogen receptor-beta sensitizes breast cancer cells to the anti-estrogenic actions of endoxifen
Repression of known ER target genes by endoxifen is enhanced in ERβ expressing cells. Parental MCF7 and MCF7-ERβ cells were treated as indicated for 24 hours. Real-time RT-PCR analysis was performed to detect expression levels of (A) cyclin D1 (CCND1), (B) PS2, (C) progesterone receptor (PR) and (D) amphiregulin (AREG). * denotes significance at the P < 0.05 level (ANOVA) compared to vehicle, # compared to estrogen treatment and δ for a given treatment between the two cell lines.