Figure 8
From: Mechanisms of suberoylanilide hydroxamic acid inhibition of mammary cell growth
Analysis of Rb2/p130 subcellular localization and interaction with E2F-4 in control and SAHA treated TM10 and TM2H cell lines. (A) Immunofluorescent staining of Rb2/p130 in TM10 and TM2H cell lines at control (C) and treated with 2.5 μM SAHA for 24 h (T). (B) Nuclear protein extracts (200 μg) of both cell lines and at the same conditions as (A) were immunoprecipitated with anti-E2F-4 polyclonal antibody followed by immunoblotting with mouse anti-Rb2/p130 monoclonal antibody. I.P., immunoprecipitation; W.B., western blot.