Figure 3
From: α-Tocopheryloxyacetic acid: a novel chemotherapeutic that stimulates the antitumor immune response
Effect of α-TEA on regulatory T cells (Treg) in the tumor microenvironment. BALB/c mice with established 4T1 mammary tumors (day 10) received oral α-TEA in their diet (~6 mg/day). On day 18 (D18) and day 26 (D26) post-tumor injection, TICs were isolated and analyzed by polychromatic flow cytometry. (A) After applying a live cell gate, CD3+ cells were delineated by the R1 gate. Subsequently, CD4+ T cells (R2) were further interrogated for CD25 and Foxp3 expression. Tregs were identified as CD3+CD4+CD25+Foxp3+ cells. Representative flow cytometric analyses for D18 are shown. (B) Treg frequency. (C) Ratio of Treg to either non-Treg CD4+ (CD4+Foxp3-) or CD8+ T cells. TICs were analyzed from three individual mice from each of two independent experiments. Subphenotype frequencies are based on all live tumor-infiltrating cells.