Figure 2From: Anterior gradient-2 plays a critical role in breast cancer cell growth and survival by modulating cyclin D1, estrogen receptor-α and survivinAGR2 knockdown reduces cell proliferation in ER-positive breast cancer cells. Cell-cycle profiles were analyzed with BrdU incorporation. Cells were pulse-labeled with 10 μM BrdU 48 hours after transfection and analyzed for BrdU incorporation with FACS. Cells were gated on Sub G1, G0/G1, S, and G2/M populations. (a) T47 D, (b) ZR-75-1, (c) MDA-MB-231, and (d) SK-BR-3 cells.Back to article page