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Figure 3 | Breast Cancer Research

Figure 3

From: Inhibition of phosphatidylcholine-specific phospholipase C downregulates HER2 overexpression on plasma membrane of breast cancer cells

Figure 3

D609-induced PC-PLC inhibition and HER2 downmodulation in SKBr3 cells. (a) Amplex Red® assay SKBr3 cells after exposure to the PC-PLC inhibitor D609. Relative PC-PLC activity was measured in the presence of D609 (50 μg/mL, black columns) and compared with that of untreated control cells (t = 0 taken as 1.0 or t = 72 hours, white columns). Histograms represent the mean ± SD (n = 4). (b) CLSM analyses of unfixed SKBr3 cells cultured in complete medium (t = 0) and in presence of D609 for the indicated time periods. After washing, cells were stained with α-PC-PLC (green) and α-HER2 (red). Nuclei were stained with DAPI (blue). Colocalization areas are represented in yellow. Scale bars, 5 μm. These experiments were independently repeated 3 times.

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Breast Cancer Research

ISSN: 1465-542X

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