Effects of lovastatin on breast cancer cells: a proteo-metabonomic study

Breast Cancer Research

Table 2 Intracellular concentrations (nmol/g cell weight) of ¹³ C-labeled endogenous metabolites (glycolysis and TCA cycle intermediates, glucose) and lipid metabolites (choline-containing phospholipids, cholesterol)

The values were calculated based on MDAMB468 cell extracts as assessed by ¹H-NMR and ¹³C-NMR. The cells were incubated with 8 μg/mL lovastatin lactone or hydroxy acid for 48 hours. Values are presented as means ± standard deviation of three independent experiments. Significance levels: * P < 0.05; ** P < 0.005; *** P < 0.001 were determined by analysis of variance (with post-hoc pairwise multiple comparison Tukey-test). chol, cholesterol; choline-PL, choline-containing phospholipids; glu, glutamate; glycolysis int, glycolysis intermediates: ¹³C-lactate+¹³C-alanine, TCA int: TCA cycle intermediates: (C2 + C3 + C4)-glutamate + (C2 + C3)-glutamine.

ISSN: 1465-542X