Figure 6

BU-32 downregulates the expression of genes involved in angiogenesis. Expression levels of vascular endothelial growth factor (VEGF), VEGF-receptor 1 (FLT1), kinase-insert domain-containing receptor (KDR), Ang1, Ang2, Tie1, Tie2, and epidermal growth factor receptor (EGFR) genes were measured by RT-PCR in (a) MDA-MB-231, (b) MCF7 and (c) SKBR3 breast cancer cell lines exposed to proteasome inhibitors BU-32 and Bortezomib, as evaluated by real-time RT-PCR. The RT-PCR was performed on 200 ng total RNA extracted with Trizol reagent from breast cancer cell lines and was cultured for 24 hours with 5 nM concentrations of Bortezomib and BU-32. Results are mean ± standard deviation of three independent determinations.