Figure 1

Growth inhibition of MCF-7 cells by decursin. (a) Chemical structure of pyranocoumarin compounds isolated from Angelica gigas root. (b) Growth inhibitory effects of decursin on exponentially growing MCF-7 cells (30–40% confluent) in T25 flasks. Exposure to different decursin concentrations (D5 μM, D20 μM, D50 μM, D100 μM) was in complete medium for 3 days. Results based on two separate experiments (mean ± standard deviation). *P < 0.05, **P < 0.001 versus control. (c) Inhibition of long-term colony growth of MCF-7 cells. Sparsely seeded cells (~10% confluent) in six-well plates were exposed to different decursin concentrations (D20 μM, D50 μM, D100 μM) in the complete medium for 7 days. After fixation with 1% glutaraldehyde, the cells remaining attached were stained in situ with 0.02% crystal violet and were photographed. DMSO, dimethylsulfoxide.