Figure 1
From: Telomeric DNA induces apoptosis and senescence of human breast carcinoma cells
T-oligos localize to nuclei. MCF-7 and normal mammary epithelial (NME) cells were supplemented with fluorescein phosphoramidite (FAM) labeled GTTAGGGTTAG and uptake was determined 30 to 60 minutes after supplementation. Multicolor fluorescent microscopy showed that FAM-labeled T-oligos accumulated in the nucleus as determined by colocalization of propidium iodide (PI) nuclear staining (red) with FAM (green), producing an orange color. As expected, no green fluorescence was observed in control, diluent-treated cells.