Figure 5

Flow cytometric analysis using A2L2 cells of serum from mice vaccinated with SINCP and Adeno. Naive mice were vaccinated with SINCP-neu (a plasmid containing Sindbis virus genes and the gene for rat HER2/neu) as the prime and twice, at 2-week intervals, with Adeno-neu (an E1,E2a-deleted adenovirus also containing the gene for rat HER2/neu) as the boost; or they were vaccinated with SINCP-βgal as the prime and then twice, at 2-week intervals, with Adeno-empty (adenovirus lacking an inserted gene) as the boost. (a) Immune serum collected 2 weeks after the second Adeno vaccination was diluted 1:100 and analyzed by flow cytometry. (b) A2L2 cells treated with fluorescein-isothiocyanate-labeled goat antimouse IgG (secondary antibody) and serum collected before vaccination (prebleed) served as the negative controls, and A2L2 cells treated with a commercial polyclonal antibody against p185 served as the positive control.