Figure 2

Expression of amino-terminal ezrin significantly reduces lung metastases of carcinoma cells. Metastatic AC2M2 cells transfected with empty pCB6 vector or a vector encoding wild-type ezrin or amino-terminal ezrin were injected (7.5 × 10³/mouse) into the mammary fat pad of syngeneic mice, and metastasis was assessed 6 weeks later, as described in the text. Images are shown from tissue sections from the lungs of mice injected with AC2M2 cells transfected with (a) empty pCB6 vector, (b) wild-type ezrin (WTC4), or (c,d) amino-terminal ezrin, stained with haematoxylin and eosin. Normal lung tissue is indicated by 'NL'. Tumour metastases are indicated by 'T'. Arrows indicate endothelial lining of vascular channels with tumour emboli. Original magnifications: panels a–c, 200×; panel d, 400×.