Table 1 Panel for flow cytometry
Antigen | Human PBMC | Mouse tumours | ||
|---|---|---|---|---|
Fluorophore | Laser channel | Fluorophore | Laser channel | |
CD3 | APC Cy 7 | R780/60 | FITC | B530/30 |
CD4 | PacBlue | V450/50 | APC + H7 | R780/60 |
CD8 | V 500 | V525/50 | APC | R670/14 |
CD11b | n/a | n/a | AF700 | R730/45 |
CD11c | APC | R670/14 | BV421 | V450/50 |
CD14 | FITC | B530/30 | n/a | n/a |
CD19 | PE | YG586/15 | BB700 | B710/50 |
CD25 | n/a | n/a | BV510 | V525/50 |
CD45 | PerCP/Cy5.5 | B710/50 | BV605 | V610/20 |
CD127 | n/a | n/a | PE | YG586/15 |
F4/80 | n/a | n/a | PECy5 | YG670/30 |
L/D | Texas red | Y610/20 | Texas red | Y610/20 |