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Fig. 4 | Breast Cancer Research

Fig. 4

From: Oestrogen deprivation induces chemokine production and immune cell recruitment in in vitro and in vivo models of oestrogen receptor-positive breast cancer

Fig. 4

NSAIDs reduce expression of CCL5 and reduce migration of PBMCs towards oestrogen-deprived MCF-7 cells. a MCF-7 cells were plated with (+E2) or without (− E2) 1 × 10−9 M oestradiol, treated with aspirin (1 mM) and mRNA expression of CCL5 was determined by RT-qPCR at 0, 24, 72 and 120 h. CCL5 expression was normalised to reference genes FKBP15 and PUM1. A transwell assay was used to measure migration of PBMCs to MCF-7 cells treated with either b 1 mM aspirin (ASA) or c 10 µM celecoxib (CBX). Migration towards oestrogen supplemented (+E2) MCF-7 cells and control media (− E2) was also measured. PHA = conditioned media from PBMCs treated with phytohaemagglutinin. Data were normalised to the complete media only control (Media). Error bars are SEM based on nine biological replicates performed across three independent experiments. P-values were calculated using unpaired t-tests

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