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Fig. 3 | Breast Cancer Research

Fig. 3

From: Oestrogen deprivation induces chemokine production and immune cell recruitment in in vitro and in vivo models of oestrogen receptor-positive breast cancer

Fig. 3

Oestrogen deprivation of MCF-7 cells alters PBMC migration in vitro. a A transwell assay was used to measure migration of PBMCs towards oestrogen-deprived MCF-7 cells treated CXC-chemokine binding protein (PVNZ 112.3) or CC-chemokine binding protein (PVNZ 112.6). Migration towards oestrogen supplemented (+E2) MCF-7 cells and control media with MCF-7 cells (− E2) was also measured. Data were normalised by dividing values from each experiment by the mean of that experiment. PHA = conditioned media from PBMCs treated with phytohaemagglutinin, a T-cell mitogen. Media = complete media only. Cells from part A were analysed by FACS for expression of immune markers CD4 (b), CD11c (c) and CD14 (d). Representative flow cytometry plots are shown in (e). Error bars are SEM based on nine biological replicates performed across three independent experiments. P-values were calculated using unpaired t-tests

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