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Fig. 4 | Breast Cancer Research

Fig. 4

From: TAZ maintains telomere length in TNBC cells by mediating Rad51C expression

Fig. 4

Knockdown of TAZ results in the deprotection of telomeres and the activation of DDR. A Western blot analysis of shelterin proteins in TAZ-depleted MDA-MB-231 cells with long-term approaches (infected with shRNA and cultured > 6 passages). Quantification of Western blots is shown in Additional file 1: Fig. S5G. B Western blot analysis of shelterin proteins in long-term TAZ knockdown (infected with shRNA and cultured > 6 passages) BT549 cells. Quantification of Western blots is shown in Additional file 1: Fig. S5H. C Immunofluorescence assay of TRF2 in BT549 and MDA-MB-231 cells treated as indicated. D Western blot analysis of the DDR pathway proteins in MDA-MB-231 and BT549 cell with long-term TAZ knockdown. Quantification of Western blots is shown in Additional file 1: Fig. S5I. E Immunofluorescence assay of p-BRCA1 in BT-549 cells with long-term TAZ knockdown. F IF-FISH assays stained with 53BP1 antibody and Cy3-conjugated telomere PNA probes were performed in the indicated BT549 cells. Co-localizing events indicate telomere dysfunction induced foci (TIFs). The percentage of cells with more than 3 TIFs were analyzed. 100 cells were counted for each experiment. G Immunofluorescence assays of TRF2 and γ-H2AX in the indicated BT549 cells. Co-localizing events indicate telomere dysfunction induced foci (TIFs). The percentage of cells with more than 3 TIFs were analyzed. 100 cells were counted for each experiment

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