Fig. 6
The different versions of the MMTV-PyMT syngeneic model respond differently to immune checkpoint blockade. Tumors from the autochthonous MMTV-PyMT model were harvested and single-cell suspensions were generated. Cells (1E6, 1E5, or 1E4) were injected into the mammary fat pad of FVB/NJ wild-type mice. When the tumors reached 100 mm3, mice were randomized into four treatment groups: vehicle (IgG2B), anti-PD-L1, anti-CTLA-4, and the combination of anti-PD-L1 and anti-CTLA-4. Tumor volumes were measured and plotted as the mean total tumor burden ± SEM (left) and survival analysis of mice are shown (right). Shown is a representative experiment of two individual experiments n=3–7/treatment groups for the 1E6 (a), 1E5 (b), 1E4 (c), and transgenic models (d). In Fig. 6d, all mice were sacrificed on the same day due to meeting endpoint criteria. Two-way ANOVA multiple comparisons and Gehan-Breslow-Wilcoxon tests were performed. *P<0.05, **P<0.01, ***P<0.001, and ****P<0.001