Fig. 6

OSM-induced LOXL2 promotes invasion in 3D extracellular matrix. a MCF7-GFP cells were seeded into a 1.5 mg/mL collagen I solution and molded into wells of a 96-well plate containing “cell-free zones.” Cells were supplemented with estrogen (50 ng/mL) and treated with OSM, OSM with βAPN (500 μM), or OSM with PXS-5120A (200 nM). Fluorescent images were taken at day 0, and at the conclusion of the experiment on day 5. We observed an increase in 3D invasion of MCF7 cells treated with OSM that was limited by the inhibition of LOXL2 enzymatic function using βAPN or PXS-5120A. Scale bar = 1,000 μm. b Graph represents the total number of MCF7-GFP cells that invaded into the cell-free space by day 5, with the various treatments discussed above. OSM treatment significantly increases 3D invasion in MCF7 cells and that is significantly reversed by LOXL2 inhibition. (Experiment n = 3; ***p < 0.001; one-way ANOVA)