Skip to main content
Fig. 2 | Breast Cancer Research

Fig. 2

From: Novel mechanism for OSM-promoted extracellular matrix remodeling in breast cancer: LOXL2 upregulation and subsequent ECM alignment

Fig. 2

OSM promotes LOXL2 protein expression. All experiments and results pertain to immunoblot assays run with 10–20 μg total protein. a MCF7 breast cancer cells were treated with OSM, IL-6, LIF, and IL-1β for 24 h. Our analysis showed that only OSM and IL-1β promoted a significant upregulation of LOXL2 protein expression. b In analyzing MDA-MB-468 breast cancer cells, the same treatments that are described above were utilized. We observed that only cells treated with OSM had significantly induced LOXL2 protein expression. c BT474 breast cancer cells, under the same conditions, showed a significant increase in LOXL2 expression with OSM and LIF treatments. d In Sk-Br-3 breast cancer cells, we observed a significant increase in LOXL2 expression with OSM and IL-6 treatment. e We again used the same treatments in MDA-MB-231 breast cancer cells. LOXL2 expression was not significantly affected by either OSM, IL-6, LIF, or IL-1β treatment after 24 h. f Relative LOXL2 protein expression was compared among three breast cancer cell lines treated with OSM. From least invasive (MCF7) to the most (MDA-MB-231), we observed a stepwise increase in LOXL2 protein expression. OSM treatment bridges LOXL2 expression between cells. g MCF7 cells were treated for 24 h with OSM; OSM, IL-6, and LIF for LOX expression. No changes are observed in lysyl oxidase expression; LOXL1 is constitutively expressed. (All experiments n = 3+; n.s. p > 0.05, **p < 0.01, ***p < 0.001; Students t test)

Back to article page