Skip to main content
Fig. 1 | Breast Cancer Research

Fig. 1

From: Serum exosomal-annexin A2 is associated with African-American triple-negative breast cancer and promotes angiogenesis

Fig. 1

Expression of AnxA2 in exosomes derived from serum samples of breast cancer patients. a Size analysis of exosomes. Representative image of the average size of exosomes isolated from serum samples of cancer patients were analyzed by Malvern Zetasizer. Studied exosomes range of size is 52.06–122.3 nm in diameter with an average size of 87.85 ± 21.30 nm. b Western blot analysis for the expression of AnxA2 and exosomal markers CD9, TSG101, and flotillin-1 in lysates of exosomes purified from breast cancer patients and non-cancer patients. MDA-MB-231 cell lysate was used as a positive control for the expression of AnxA2, CD9, TSG101, flotillin-1, and calnexin. Calnexin (endoplasmic reticulum marker) was analyzed as a marker absent in exosomes. Coomassie blue staining was performed as an equal loading control for exosomes derived from serum samples of patients. c Exosomes purified from serum samples of breast cancer patients were immunoprecipitated with an antibody to AnxA2, EpCAM, or isotype mouse/rabbit immunoglobulin (M-IgG/ Rb-IgG). The immunoprecipitated whole exosomes were lysed with RIPA lysis buffer and analyzed for the expression of AnxA2, EpCAM (endothelial marker), exosomal markers (CD9, TSG101, and flotillin-1), calnexin (endoplasmic reticulum marker), and GM130 (cis-Golgi marker) by Western blot analysis. Calnexin and GM130 were used as a negative control for exosomes

Back to article page