| B7–1 | B7–2 | ICAM-1 | MHCI | MHCII | FasR |
4T1 |
 Non-irradiated | 6.7 ± 1.3a | 28.6 ± 6.6 | 20.6 ± 2.2a | 25.2 ± 3.4a | 43.4 ± 1.8 | 405.3 ± 20.4 |
 Irradiated | 14.8 ± 2.8ab | 50.2 ± 1.4 | 35.7 ± 4.0c | 25.3 ± 3.6a | 133.3 ± 4.2 | 1073.0 ± 71.6 |
 % change | 150 ± 47.1 | 57.1 ± 19.7 | 79.3 ± 3.5 | 11.7 ± 15.4 | 209.6 ± 31.4 | 171.8 ± 6 |
EMT6 |
 Non-irradiated | 61.3 ± 7.2ab | 75 ± 5.7 | 22.0 ± 3.0ab | 16.6 ± 2.3 | 94.3 ± 3.6 | 2396.0 ± 159.0 |
 Irradiated | 263.6 ± 45.1 | 93.1 ± 5.3 | 30.5 ± 4.0bc | 49.9 ± 3.3 | 164.1 ± 12.5 | 4688.2 ± 99.9 |
 % change | 356.6 ± 7 | 19.1 ± 13.5 | 22.5 ± 8.5 | 203 ± 36.1 | 76.8 ± 2.8 | 102.7 ± 7.5 |
- The 4T1 and EMT6 cells were exposed to 0 Gy (non-irradiated) or 100 Gy. At 24 h after irradiation, cells were harvested and stained with fluorochrome conjugated anti-CD80 (B7–1), anti-CD86 (B7–2), anti-H2-Kb (MHC I), anti-I-Ad/I-Ed (MHC II), anti-CD54 (ICAM-1), and anti-CD95 (FasR). The samples were analyzed on a multiparameter flow cytometer (FACSCantoII). The differences in mean fluorescence intensities, ΔMFI, between unstained and stained non-irradiated cells, and unstained and stained irradiated cells were tabulated. The experiment was repeated twice and one representative experiment with triplicates is shown. The results are represented as ΔMFI ± standard deviation. Means with the same letter are not statistically significant from each other (p > 0.05, two-way analysis of variance followed by Tukey’s multiple comparison)