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Table 1 Differences in expression of MHC and costimulatory molecules in non-irradiated and irradiated breast cancer cells

From: Tumor-derived granulocyte colony-stimulating factor diminishes efficacy of breast tumor cell vaccines

 

B7–1

B7–2

ICAM-1

MHCI

MHCII

FasR

4T1

 Non-irradiated

6.7 ± 1.3a

28.6 ± 6.6

20.6 ± 2.2a

25.2 ± 3.4a

43.4 ± 1.8

405.3 ± 20.4

 Irradiated

14.8 ± 2.8ab

50.2 ± 1.4

35.7 ± 4.0c

25.3 ± 3.6a

133.3 ± 4.2

1073.0 ± 71.6

 % change

150 ± 47.1

57.1 ± 19.7

79.3 ± 3.5

11.7 ± 15.4

209.6 ± 31.4

171.8 ± 6

EMT6

 Non-irradiated

61.3 ± 7.2ab

75 ± 5.7

22.0 ± 3.0ab

16.6 ± 2.3

94.3 ± 3.6

2396.0 ± 159.0

 Irradiated

263.6 ± 45.1

93.1 ± 5.3

30.5 ± 4.0bc

49.9 ± 3.3

164.1 ± 12.5

4688.2 ± 99.9

 % change

356.6 ± 7

19.1 ± 13.5

22.5 ± 8.5

203 ± 36.1

76.8 ± 2.8

102.7 ± 7.5

  1. The 4T1 and EMT6 cells were exposed to 0 Gy (non-irradiated) or 100 Gy. At 24 h after irradiation, cells were harvested and stained with fluorochrome conjugated anti-CD80 (B7–1), anti-CD86 (B7–2), anti-H2-Kb (MHC I), anti-I-Ad/I-Ed (MHC II), anti-CD54 (ICAM-1), and anti-CD95 (FasR). The samples were analyzed on a multiparameter flow cytometer (FACSCantoII). The differences in mean fluorescence intensities, ΔMFI, between unstained and stained non-irradiated cells, and unstained and stained irradiated cells were tabulated. The experiment was repeated twice and one representative experiment with triplicates is shown. The results are represented as ΔMFI ± standard deviation. Means with the same letter are not statistically significant from each other (p > 0.05, two-way analysis of variance followed by Tukey’s multiple comparison)