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Fig. 7 | Breast Cancer Research

Fig. 7

From: Tumor-derived granulocyte colony-stimulating factor diminishes efficacy of breast tumor cell vaccines

Fig. 7

Comparison of immune cell subsets in draining lymph nodes (DLNs) from mice bearing 4T1 and 4T1.G-CSF− and naïve mice. Female balb/cByJ mice (n = 3) received subcutaneous injections of 1 × 106 4T1 or 4T1.G-CSF− cells. Once tumors reached 500–700 mm3, DLNs were harvested and single cell suspensions obtained. Leukocytes isolated from naïve mice served as control (n = 3). Flow cytometric analysis was performed to determine the percentage of myeloid derived suppressor cells (MDSCs) (CD11b+Ly6G+Ly6C+), B cells (CD19+), T cells (CD3+) and regulatory T cells (Tregs) (CD4+CD25+FoxP3+). Absolute numbers of MDSCs (a), T cells (b), B cells (c), and Tregs (d) were quantified and results are represented as mean ± standard error (*p < 0.05, Kruskal-Wallis test with Dunn’s post-hoc test)

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