Fig. 6
Comparison of spleen size, spleen weight and immune cell subsets in spleen of 4T1 and 4T1.G-CSF− tumor-bearing mice and naïve mice. Female balb/cByJ mice received subcutaneous injections of 1 × 106 4T1 or 4T1.G-CSF− cells. Spleens were harvested when tumor volumes reached 500–700 mm3. a Spleens from representative mice bearing (1) 4T1, (2) 4T1.G-CSF− tumors and from (3) a naïve mouse. b Spleen weight at the time of harvest. The spleens were processed and flow cytometric analysis was performed to determine the percentage of myeloid derived suppressor cells (MDSCs) (CD11b+Ly6G+Ly6C+), B cells (CD19+), T cells (CD3+) and regulatory T cells (Tregs) (CD4+CD25+FoxP3+). Absolute numbers of MDSCs (c), T cells (d), B cells (e), and Tregs (f) were quantified and results are presented as mean ± standard error (*p < 0.05, Kruskal-Wallis test with Dunn’s post-hoc test)