Fig. 7From: Consistent and reproducible cultures of large-scale 3D mammary epithelial structures using an accessible bioprinting platformBreast epithelial morphology in human collagen. (a) Bioprinted red fluorescent protein (RFP) and green fluorescent protein (GFP) MCF12A cell deposits in human collagen gel 2 days after printing. (b) GFP (b1), RFP (b2), and merged (b3 and b4) images of organoid formed from alternating prints of GFP and RFP MCF12A cells in human collagen 7 days after printing; higher magnification image (b4) demonstrates mix of GFP+ and RFP+ cells in the same structure. c) Immunofluorescent staining of a cross-section of the organoid in (b) with 4′,6-diamidino-2-phenylindole (DAPI) (c1; blue), GFP (c2; green), and RFP (c3; red). Merged image (c4) demonstrates presence of both cell types in the same organoid structure. Scale bars: a = 200 μm, b = 500 μm, c = 100 μMBack to article page