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Fig. 7 | Breast Cancer Research

Fig. 7

From: Consistent and reproducible cultures of large-scale 3D mammary epithelial structures using an accessible bioprinting platform

Fig. 7

Breast epithelial morphology in human collagen. (a) Bioprinted red fluorescent protein (RFP) and green fluorescent protein (GFP) MCF12A cell deposits in human collagen gel 2 days after printing. (b) GFP (b1), RFP (b2), and merged (b3 and b4) images of organoid formed from alternating prints of GFP and RFP MCF12A cells in human collagen 7 days after printing; higher magnification image (b4) demonstrates mix of GFP+ and RFP+ cells in the same structure. c) Immunofluorescent staining of a cross-section of the organoid in (b) with 4′,6-diamidino-2-phenylindole (DAPI) (c1; blue), GFP (c2; green), and RFP (c3; red). Merged image (c4) demonstrates presence of both cell types in the same organoid structure. Scale bars: a = 200 μm, b = 500 μm, c = 100 μM

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