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Fig. 5 | Breast Cancer Research

Fig. 5

From: Consistent and reproducible cultures of large-scale 3D mammary epithelial structures using an accessible bioprinting platform

Fig. 5

Alternated prints form contiguous organoids with intermingled cells. Alternating red fluorescent protein (RFP)- and green fluorescent protein (GFP)-labeled MCF12A cells. (a and b) Examples of organoids resulting from alternating prints of RFP+ and GFP+ MCF12A cells. Forty GFP+ and RFP+ MCF12A cells were printed with 200-μm spacing, and cells were grown and imaged at (a) day 7 and (b) day 9. Scale bar: 200 μm. (c) Immunofluorescence staining of CX32 (red) and GFP (green) in cross-section of an organoid formed as described in (a and b). Presence of GFP+ cells intermingled along the same lumen as GFP cells with expression of CX32 along cell boundaries indicates that the cells from adjacent print sites intermingled and formed cellular junctions with cells from neighboring prints to form contiguous structures Scale bars: a and b = 500 μm; c = 50 μm

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